東京大学物性研究所・井上研究室

研究業績

原著論文

  1. Pro219 is an Electrostatic Color Determinant in the Light-driven Sodium Pump KR2
    Yuta Nakajima†, Laura Pedraza-González†, Leonardo Barneschi, Keiichi Inoue, Massimo Olivucci*, Hideki Kandori*. (2021) Communications Biology, 4, Article number: 1185
    doi: 10.1038/s42003-021-02684-z (†: Equally contributed)
  2. Thermostable Light-Driven Inward Proton Pump Rhodopsins
    Yuma Kawasaki, Masae Konno, Keiichi Inoue*. (2021) Chemical Physics Letters, 779, Article number: 138868
    doi: 10.1016/j.cplett.2021.138868
  3. Crystal Structure of Schizorhodopsin Reveals Mechanism of Inward Proton Pumping
    Akimitsu Higuchi, Wataru Shihoya, Masae Konno, Tatsuya Ikuta, Hideki Kandori, Keiichi Inoue*, Osamu Nureki*. (2021) Proceedings of the National Academy of Sciences of the United States of America, 118, issue 14, Article number: e2016328118
    doi: 10.1073/pnas.2016328118
    Press release
  4. Exploration of Natural Red-shifted Rhodopsins Using a Machine Learning-based Bayesian Experimental Design
    Keiichi Inoue*, Masayuki Karasuyama, Ryoko Nakamura, Masae Konno, Daichi Yamada, Kentaro Mannen, Takashi Nagata, Yu Inatsu, Hiromu Yawo, Kei Yura, Oded Béjà, Hideki Kandori, Ichiro Takeuchi*. (2021) Communications Biology, 4, Article number: 362
    doi: 10.1038/s42003-021-01878-9
  5. TAT Rhodopsin is an Ultraviolet-Dependent Environmental pH Sensor
    Chihiro Kataoka, Teppei Sugimoto, Shunta Shigemura, Kota Katayama, Satoshi P. Tsunoda, Keiichi Inoue, Oded Béjà, Hideki Kandori*. (2021) Biochemistry, in press
    doi: 10.1021/acs.biochem.0c00951
  6. Active Learning for Level Set Estimation under Input Uncertainty and Its Extensions
    Yu Inatsu, Masayuki Karasuyama, Keiichi Inoue, Ichiro Takeuchi*. (2020) Neural Computation, 32, issue 12, pp 2486-2531
    doi: 10.1162/neco_a_01332
  7. Active Learning of Bayesian Linear Models with High-Dimensional Binary Features by Parameter Confidence-Region Estimation
    Yu Inatsu, Masayuki Karasuyama, Keiichi Inoue, Hideki Kandori, Ichiro Takeuchi*. (2020) Neural Computation, 32, issue 10, pp 1998-2031
    doi: 10.1162/neco_a_01310
  8. Excitonic Coupling Effect on the Circular Dichroism Spectrum of Sodium-pumping Rhodopsin KR2
    Kazuhiro J. Fujimoto*, Keiichi Inoue. (2020) Journal of Chemical Physics, 153, Article number: 045101
    doi: 10.1063/5.0013642
  9. Gate-keeper of Ion Transport ―A Highly Conserved Helix-3 Tryptophan in A Channelrhodopsin Chimera, C1C2/ChRWR
    Yujiro Nagasaka†, Shoko Hososhima†, Naoko Kubo, Takashi Nagata, Hideki Kandori, Keiichi Inoue and Hiromu Yawo*. (2020) Biophysics and physicobiology (BPPB), 17, pp 59-70
    doi: 10.2142/biophysico.BSJ-2020007 (†: Equally contributed)
  10. Schizorhodopsins: A Family of Rhodopsins from Asgard Archaea that Function as Light-driven Inward H+ Pumps
    Keiichi Inoue*, Satoshi P. Tsunoda, Manish Singh, Sahoko Tomida, Shoko Hososhima, Masae Konno, Ryoko Nakamura, Hiroki Watanabe, Paul-Adrian Bulzu, Horia L. Banciu, Adrian-Ştefan Andrei, Takayuki Uchihashi, Rohit Ghai, Oded Béjà, Hideki Kandori*. (2020) Science Advances, 6, No. 15, Article number: eaaz2441
    doi: 10.1126/sciadv.aaz2441
  11. Infrared Spectroscopic Analysis on Structural Changes around the Protonated Schiff base upon Retinal Isomerization in Light-driven Sodium Pump KR2
    Sahoko Tomida, Shota Ito, Tomoya Mato, Yuji Furutani, Keiichi Inoue, Hideki Kandori*. (2020) Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1861, issue 7, Article number: 148190
    doi: 10.1016/j.bbabio.2020.148190
  12. Physical Disuse Contributes to Widespread Chronic Mechanical Hyperalgesia, Tactile Allodynia, and Cold Allodynia through Neurogenic Inflammation and Spino-Parabrachio-Amygdaloid Pathway Activation
    Yusuke Ohmichi†,*, Mika Ohmichi†, Ryoichi Tashima, Koji Osuka, Kaori, Fukushige, Dominika Kanikowska, Yugo Fukazawa, Hiromu Yawo, Makoto Tsuda, Munekazu Naito, Takashi Nakano. (2020) Pain, 161, issue 8, pp 1808-1823
    doi: 10.1097/j.pain.0000000000001867. (†: Equally contributed)
  13. Allosteric Communication to the Retinal Chromophore upon Ion Binding in a Light-driven Sodium Ion Pumping Rhodopsin
    Akihiro Otomo, Misao Mizuno, Keiichi Inoue, Hideki Kandori, Yasuhisa Mizutani*. (2020) Biochemistry, 59, issue 4, pp 520-529
    doi: 10.1021/acs.biochem.9b01062
  14. Optogenetic Analysis of Respiratory Neuronal Networks in The Ventral Medulla of Neonatal Rats Producing Channelrhodopsin in Phox2b-Positive Cells
    Keiko Ikeda, Hiroyuki Igarashi, Hiromu Yawo, Kazuto Kobayashi, Satoru Arata, Kiyoshi Kawakami, Masahiko Izumizaki, Hiroshi Onimaru. (2019) Pflügers Arch, 471, issue 11-12, pp 1419-1439
    doi: 10.1007/s00424-019-02317-9
  15. Crystal Structure of Heliorhodopsin
    Wataru Shihoya, Keiichi Inoue, Manish Singh, Masae Konno, Shoko Hososhima, Keitaro Yamashita, Kento Ikeda, Akimitsu Higuchi, Tamaki Izume, Sae Okazaki, Masanori Hashimoto, Ritsu Mizutori, Sahoko Tomida, Yumeka Yamauchi, Rei Abe-Yoshizumi, Kota Katayama, Satoshi P. Tsunoda, Mikihiro Shibata, Yuji Furutani, Alina Pushkarev, Oded Béjà, Takayuki Uchihashi, Hideki Kandori*, Osamu Nureki*. (2019) Nature, 574, issue 7776, pp 132-136
    doi: 10.1038/s41586-019-1604-6
  16. Unique Photochemistry Observed in a New Microbial Rhodopsin
    Chihiro Kataoka, Keiichi Inoue, Kota Katayama, Oded Béjà, Hideki Kandori*. (2019) The Journal of Physical Chemistry Letters, 10, pp 5117-5121
    doi: 10.1021/acs.jpclett.9b01957
  17. X-ray Crystallographic Structure and Oligomerization of Gloeobacter Rhodopsin
    Takefumi Morizumi†, Wei-Lin Ou†, Ned Van Eps, Keiichi Inoue, Hideki Kandori, Leonid S. Brown, Oliver P. Ernst*. (2019) Scientific Reports, 9, Article number: 11283
    doi: 10.1038/s41598-019-47445-5 (†: Equally contributed)
  18. Engineered Functional Recovery of Microbial Rhodopsin without Retinal-Binding Lysine
    Yumeka Yamauchi, Masae Konno, Daichi Yamada, Kei Yura, Keiichi Inoue, Oded Béjà, Hideki Kandori*. (2019) Photochemistry and Photobiology, 95, pp 1116-1121
    doi: 10.1111/php.13114
  19. Red-shifting Mutation of Light-driven Sodium Pump Rhodopsin
    Keiichi Inoue, María del Carmen Marín, Sahoko Tomida, Ryoko Nakamura, Yuta Nakajima, Massimo Olivucci, Hideki Kandori*. (2019) Nature Communications, 10, Article number: 1993
    doi: 10.1038/s41467-019-10000-x
  20. Casting Light on Asgardarchaeota Metabolism in a Sunlit Microoxic Niche
    Paul-Adrian Bulzu†, Adrian-Ştefan Andrei†, Michaela M. Salcher, Maliheh Mehrshad, Keiichi Inoue, Hideki Kandori, Oded Béjà, Rohit Ghai*, Horia L. Banciu. (2019) Nature Microbiology, 4, issue 7, pp 1129-1137
    doi: 10.1038/s41564-019-0404-y (†: Equally contributed)
  21. Ultrafast Dynamics of Heliorhodopsins
    Shinya Tahara, Manish Singh, Hikaru Kuramochi, Wataru Shihoya, Keiichi Inoue, Osamu Nureki, Oded Béjà, Yasuhisa Mizutani, Hideki Kandori, Tahei Tahara*. (2019) The Journal of Physical Chemistry B, 123, issue 11, pp 2507-2512
    doi: 10.1021/acs.jpcb.9b00887
  22. Heliorhodopsins are Absent in Diderm (Gram-negative) Bacteria: Some Thoughts and Possible Implications for Activity
    José Flores-Uribe†, Gur Hevroni†, Rohit Ghai, Alina Pushkarev, Keiichi Inoue, Hideki Kandori, Oded Béjà*. (2019) Environmental Microbiology Reports, 11, issue 3, pp 419-424
    doi: 10.1111/1758-2229.12730 (†: Equally contributed)
  23. Resonance Raman Investigation of the Chromophore Structure of Heliorhodopsins
    Akihiro Otomo, Misao Mizuno, Manish Singh, Wataru Shihoya, Keiichi Inoue, Osamu Nureki, Oded Béjà, Hideki Kandori, Yasuhisa Mizutani*. (2018) The Journal of Physical Chemistry Letters, 9, issue 22, pp 6431-6436
    doi: 10.1021/acs.jpclett.8b02741
  24. Understanding Colour Tuning Rules and Predicting Absorption Wavelengths of Microbial Rhodopsins by Data-Driven Machine-Learning Approach
    Masayuki Karasuyama†, Keiichi Inoue†, Ryoko Nakamura, Hideki Kandori*, Ichiro Takeuchi*. (2018) Scientific Reports, 8, Article number:15580
    doi: 10.1038/s41598-018-33984-w (†: Equally contributed)

総説論文

  1. Microbial Rhodopsins: The Last Two Decades
    Andrey Rozenberg†, Keiichi Inoue†, Hideki Kandori, Oded Béjà. (2021) Annual Review of Microbiology, 75, Published on the web
    doi: 10.1146/annurev-micro-031721-020452 (†: Equally contributed)

著書

  1. Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins
    Keiichi Inoue* (2021) in Optogenetics − Light-Sensing Proteins and Their Applications in Neuroscience and Beyond (eds. Hiromu Yawo, Hideki Kandori, Amane Koizumi, Ryoichiro Kageyama) pp 89-126 (Springer Nature, 2021)
    doi: 10.1007/978-981-15-8763-4_6
  2. Application of Optogenetics for Muscle Cells and Stem Cells
    Toshifumi Asano, Daniel Boon Loong Teh, Hiromu Yawo* (2021) in Optogenetics − Light-Sensing Proteins and Their Applications in Neuroscience and Beyond (eds. Hiromu Yawo, Hideki Kandori, Amane Koizumi, Ryoichiro Kageyama) pp 359-375 (Springer Nature, 2021)
    doi: 10.1007/978-981-15-8763-4_23

プロトコル論文

  1. Ion transport assay of SzRs in E. coli cells
    Masae Konno, Keiichi Inoue*, Hideki Kandori*. (2021) Bio-protocol, bio-protocol.org/prep800

解説記事

  1. 第3のロドプシン:ヘリオロドプシン
    井上 圭一 (2021) 日本化学会誌 化学と工業「ディビジョン・トピックス」, 第74巻, 第4号, pp 307
  2. 光駆動タンパク質ロドプシンの分子機能エンジニアリング
    井上 圭一 (2020) 生物工学会誌 生物工学「特集 ボトムアップ生物工学」, 第98巻, 第12号, pp 664-668
  3. Shining Light on Rhodopsin Selectivity: How Do Proteins Decide Whether to Transport H+ or Cl?
    Keiichi Inoue* (2020) The Journal of Biological Chemistry, 295, issue 44, pp 14805-14806
    doi: 10.1074/jbc.H120.016032
  4. A Series of Commentaries for A Symposium Entitled “Session 3SDA - Optogenetics: Applying Photoreceptor for Understanding Biological Phenomena”
    Satoshi P. Tsunoda*, Keiichi Inoue (2020) Biophysical Reviews, 12, issue 2, pp 295-296
    doi: 10.1007/s12551-020-00674-9
  5. ラットを用いたオプトジェネティクス(光遺伝学)
    八尾 寛、深澤有吾、冨田浩史、五十嵐敬幸 (2020) 日本実験動物協会情報誌 LABIO 21, in press
  6. 光で細胞を制御する―多様な微生物型ロドプシンとオプトジェネティクス―
    井上 圭一 (2020) 日本光学会誌 光学「光で細胞のはたらきを見る・操る」, 第49巻, 第1号, pp 2-7
  7. ハエトリグモにおける視覚機能とオプシンの吸収波長特性の関係
    永田 崇、寺北 明久 (2019) 日本比較生理生化学会誌 比較生理生化学 総説, 第36巻, 第3号, pp 175-181
  8. オプトジェネティクス触覚モデルによる異種感覚間可塑性の定量的解析
    阿部 健太、八尾 寛 (2019) 日本生物物理学会誌 生物物理誌「トピックス」, 第59巻, 第6号, pp 317-319
  9. 微生物型ロドプシンのもたらす新たな生物と光の関係とその応用
    井上 圭一 (2019) 生物工学会誌 生物工学「バイオミディア」, 第97巻, 第10号, pp 616
  10. 第3のロドプシン:ヘリオロドプシンの発見
    井上 圭一, 神取 秀樹 (2019) バイオサイエンスとインダストリー (B&I), 第77巻, 第1号, pp 42-43

受賞

  1. 日本生体エネルギー研究会第46回討論会学生ポスター発表優秀賞
    「TATロドプシンのpKaと多量体形成の制御における保存されたグルタミン酸の役割」
    萬年 健太郎永田 崇、戸叶 貴也、内橋 貴之、Oded Béjà、井上 圭一 2020年12月15日
  2. 第5回分子科学国際学術賞
    「微生物型ロドプシンの光機能発現メカニズムの研究」
    井上 圭一 2020年9月17日
  3. 第2回晝馬輝夫光科学賞
    「新奇な微生物型ロドプシンの光機能およびその光反応メカニズム」
    井上 圭一 2020年3月5日
  4. 第21回サー・マーティン・ウッド賞
    「光受容型膜タンパク質微生物型ロドプシンの生体機能発現メカニズム研究
    (The study on the mechanism of biological function of photoreceptive membrane protein, microbial rhodopsin)」
    井上 圭一 2019年11月23日
  5. 第4回日本光生物学協会奨励賞
    「微生物型ロドプシンの機能・構造・メカニズム研究と新奇分子デザイン」
    井上 圭一 2018年8月9日